No cytotoxicity of the examined substances towards peripheral blood mononuclear cells was found.Ĭholestasis is the major cause of bile acid accumulation leading to liver damage. The isolates partly reduced oxidative (oxidation of thiol groups) and nitrative (tyrosine nitration) damage to blood plasma proteins, decreased plasma lipid peroxidation as well as enhanced the non-enzymatic antioxidant capacity of blood plasma. Additionally, antioxidant actions of apiorutin, rutin, narcissin (isorhamentin 3-O-β-D-rutinoside) and licoagro-side B were assessed in human blood plasma, exposed to the peroxynitrite-induced oxidative stress in vitro.

For the first time in Herniaria genus, as well as in Caryophylaceae family the presence of apiorutin were revealed. The structures were established by extensive 1D and 2D NMR spectroscopic analyses, as well as HRESIMS data. (Caryophyllaceae) led to the identification and isolation of four known flavonoids, one known and three undescribed maltol derivatives, and benzyl β-gentiobioside. The phytochemical investigation of the whole plant extracts of Herniaria glabra L. The parameters of oxidative stress: MDA, a measure of lipid peroxidation, and inflammation parameters: C-reactive protein (CRP), fibrinogen, albumins, number of leukocytes (Leu), granulocytes ( Gr), lymphocytes (Ly) and monocytes (Mo) and biochemical markers of cholestasis: activity of ?-glutamyltransferase (?-GT) and alkaline phosphatase (AP) enzymes, the level of total, direct and indirect bilirubin were determined by standard biochemical methods. All the participants were subjected to a clinical, laboratory and ultrasonic check-up at the Internal Department of the Military Hospital in Nis. Seventy adult subjects of either sex included in the study were devided into two groups: I - 40 patients with obstructive icterus caused by choledocholithiasis, and II - 30 healthy individuals.

Therefore, the aim of this prospective study was to establish correlation of the parameters of inflammation and biochemical markers of cholestasis with the intensity of oxidative stress in pathogenesis of liver function disorders. The toxicity of MDA is based on its ability to act as a mutagenic agent in a cell. The product of oxidative stress, malondialdehyde (MDA), is extremely cytotoxic and damages cell membranes and intracellular macromolecules. During choledocholitiasis inflammatory oxidant stress involves the promotion of mitochondrial dysfunction through an intracellular oxidant stress in hepatocytes leading mainly to necrosis and less to apoptosis.

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